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Soni, K. B.
- A Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran
Abstract Views :484 |
PDF Views:171
Authors
R. S. Lekshmi
1,
K. B. Soni
1,
Swapna Alex
1,
Deepa S. Nair
1,
Lekha Sreekantan
1,
B. R. Reghunath
1
Affiliations
1 College of Agriculture, Vellayani, Thiruvananthapuram, Kerala - 695 522, IN
1 College of Agriculture, Vellayani, Thiruvananthapuram, Kerala - 695 522, IN
Source
Journal of Horticultural Sciences, Vol 11, No 2 (2016), Pagination: 116-123Abstract
A simple and rapid protocol for somatic embryogenesis in banana cv. Nendran (AAB) using immature male flowers (IMF) has been developed. The IMF produced palewhite to yellow, globular embryogenic callus on MS medium supplemented with BA (0.05 - 0.50mgL-1) and picloram (0.50 - 2.00mgL-1) with explant response of to 30 per cent. Addition of ascorbic acid (20mgL-1) and Gelrite© (0.45 per cent) to callus induction medium reduced interference from phenolic exudation. Embryogenesis was induced (33.3 to 60 per cent) on semisolid (0.30 per cent Gelrite©) MS medium supplemented with BA 2mgL-1 + IAA 0.5mgL-1. The somatic embryos showed 60-80 per cent germination on half- strength semisolid MS medium with BA 2mgL-1 + IAA 0.5mgL-1. Transfer of germinated embryos to semisolid MS medium supplemented with BA 2mgL-1 + NAA 1mgL-1 under 14 h light /8h dark photoperiod resulted in hundred percent conversion to plantlets. This protocol takes merely 6 months for producing plantlets from immature flower buds through somatic embryogenesis, without any intermediate liquid cultures.Keywords
Immature Male Flowers, Somatic Embryogenesis, Picloram, Musa spp., Nendran.Full Text
References
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- Smitha, P.D. and Ashalatha, S.N. 2011. Effect of picloram on somatic embryogenesis from leaf sheath explants in diploid Musa acuminata cv. Njalipoovan. Pl. Tiss. Cult. Biotechnol., 21(1):83-87 doi:10.3329/ptcb.v21i1.9612
- Soubir, T., Salil, K.B., Ajoy, M., Md. Sadrul, A. and Sarder, N.U. 2006. Control of phenolic compound secretion and effect of growth regulators for organ formation from Musa spp. cv. Kanthali floral bud explants. Amer. J. Biochem. Biotechnol.,2(3):97-104 doi:10.3844/ajbbsp.2006.97.104
- Characterization of novel strains of Trichoderma spp. and their utilization in management of damping off disease in tomato
Abstract Views :98 |
PDF Views:81
Authors
ATHIRA NAIR
1,
G. V. SIBLE
2,
ANIT CYRIAC
1,
SUSHA S. THARA
1,
JOY MICHAL JOHNSON
3,
N. S. RADHIKA
4,
K. B. SONI
5
Affiliations
1 Department of Plant Pathology,, IN
2 Regional Agricultural Research Station, Kumarakom, Kottayam - 686563, Kerala, India., IN
3 arming Systems Research Station, Sadanandapuram, Kollam - 691531, Kerala, India ., IN
4 Department of Plant Pathology, College of Agriculture, Padannakkad, Kasaragod - 671314, Kerala, India ., IN
5 Department of Plant Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram - 695522, Kerala, India ., IN
1 Department of Plant Pathology,, IN
2 Regional Agricultural Research Station, Kumarakom, Kottayam - 686563, Kerala, India., IN
3 arming Systems Research Station, Sadanandapuram, Kollam - 691531, Kerala, India ., IN
4 Department of Plant Pathology, College of Agriculture, Padannakkad, Kasaragod - 671314, Kerala, India ., IN
5 Department of Plant Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram - 695522, Kerala, India ., IN
Source
Journal of Biological Control, Vol 36, No 1 (2022), Pagination: 31 - 46Abstract
Chemical fungicides used in plant disease management may have deteriorative effects on humans, animals, and the environment. The use of native strains of Trichoderma spp. against plant diseases may help to reduce the dependence on chemical fungicides. In this study, eleven novel isolates of Trichoderma spp. from virgin forest soils of different agro-climatic zones of Kerala were characterized and evaluated for their efficacy against damping off disease of tomato caused by Pythium aphanidermatum under in vitro and in vivo; and also, against wilt pathogen, Fusarium oxysporum under in vitro conditions. Dual culture assay showed that all the Trichoderma isolates were found to inhibit the growth of P. aphanidermatum and F. oxysporum under in vitro conditions with multiple modes of action. The mycelial colour, texture, and conidial characters varied among all the isolates. The volatile metabolites by isolates of Trichoderma spp. also showed in vitro inhibition of the pathogens. Seed treatment (20 g kg-1) and potting medium addition @ 2 % (w/w) of isolates TRMW-2, TRKR-2, TRPN-3, TRPN-11 and TRPN-17 could effectively reduce pre- and post-emergence damping off of tomato. Among them, isolates TRMW-2, TRKR-2, and TRPN-11 were the most effective ones in reducing pre- and post-emergence damping off to about 72 and 90 percent respectively. Molecular identification of the isolates of Trichoderma spp. using ITS universal primers revealed similarity with certain reference strains of the NCBI Genbank database.Keywords
Biocontrol, damping off, Fusarium oxysporum, Pythium aphanidermatum, Trichoderma, wiltFull Text
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